New substrate for fluorometric determination of gamma-glutamyltransferase activity in serum.
نویسندگان
چکیده
We describe the preparation of a new substrate, N-gamma-L-glutamyl-5-aminoisophthalic acid dimethyl ester hydro-chloride, for the fluorometric determination of gamma-glutamyltransferase activity in serum by the "front-surface" technique. Details of the resulting method are provided. The final reaction mixture contains 4 mmol of the substrate per liter of tris(hydroxymethyl)aminomethane (100 mmol/liter) and glycylglycine (75 mmol/liter) at pH 8.2 (25 degrees C).
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 23 5 شماره
صفحات -
تاریخ انتشار 1977